AB072. Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway

© Translational Andrology and Urology. All rights reserved. Transl Androl Urol, 2015;4(S1) www.amepc.org/tau PubMed, and also report some research progress of acrosome formation in our laboratory. Results: Acrosome formation can be divided into four stages: Golgi-phase, cap-phase, acrosome-phase and maturation-phase. In the past 10 years, with gene targeting technology, more than ten genes were identified to be related acrosome formation in mice. Those genes include Casein kinase II α ́ catalytic subunit (Csnk2a2), HIV-1 Revbinding protein (Hrb), Golgi-associated PDZand coiledcoil motif-containing protein (Gopc), Beta-glucosidase 2 (Gba2), Zona pellucida binding protein 1 (Zpbp1), protein interacting with C kinase 1 (Pick1), heat shock protein 90kDa beta member 1 (Hsp90β1), autophagy-related gene 7 (Atg7), sperm acrosome associated 1 (Spaca1), Dpy19-like protein 2 (Dpy19l2) and stromal membraneassociated protein 2 (Smap2). Recently, we generated a Ccdc62 knockout mouse model with CRISPR-Cas9 system. A preliminary data showed that the male mice with Ccdc62 knockout were infertile, and 98% of sperm showed abnormal head with very lower motility, which suggested that Ccdc62 played a very important role in mouse acrosome formation. Globozoospermia is a rare type of teratozoospermia accounting for <0.1% of male infertility. It has reported that the mutation of SPATA16, PICK1 and DPY19L2 were related to clinical globozoospermia. Conclusions: The process of acrosome formation is regulated by multiple genes and its disorder will results in globozoospermia.